Journal:

Article Title: Identification of Vascular Lineage-Specific Genes by Transcriptional Profiling of Isolated Blood Vascular and Lymphatic Endothelial Cells

doi:

Figure Lengend Snippet: Blood vessel-specific expression of VEGFR-1/Flt1, N-cadherin, versican, and endoglin in normal human skin. Differential immunostains reveal mutually exclusive expression of LYVE-1 (red) on lymphatic vessels and of VEGFR-1/Flt-1 (A), N-cadherin (E), versican (I), and endoglin (M) on cutaneous blood vessels (green). Nuclear Hoechst counterstain (blue) was used to depict skin morphology. Double immunostains for the pan-endothelial marker CD31 (red) and selected blood vascular markers (green) demonstrate that only a fraction of all vessels also express VEGFR-1/Flt1 (B–D), N-cadherin (F–H), versican (J–L), or endoglin (N–P). Scale bar, 50 μm.

Article Snippet: 25 Immunostains Immunofluorescence stainings were performed on 6-μm cryostat sections of neonatal human foreskin or on 10-μm sections of mouse embryos as previously described, 22,26 using antibodies to Prox1, 22 murine or human LYVE-1 (kindly provided by Dr. D. Jackson, John Radcliffe Hospital, Oxford, UK 20 ), human CD34 and CD31 (BD Pharmingen), mouse VEGFR-1 (MF1; kindly provided by Dr. DJ Hicklin, Imclone Systems, New York, NY) and human VEGFR-1/Flt-1 (Sigma), N-cadherin (Transduction Laboratories, San Diego, CA), versican (clone 12C5; Developmental Studies Hybridoma Bank, University of Iowa, Ames, IA), mouse endoglin (BD Pharmingen) and human endoglin (Neomarkers, Fremont, CA), macrophage mannose receptor (BD Biosciences), desmoplakin (Serotec, Raleigh, NC), MIP-3-α (Santa Cruz Biotechnology, Santa Cruz, CA), and corresponding secondary antibodies labeled with AlexaFluor488 or AlexaFluor594 (Molecular Probes, Eugene, Oregon).

Techniques: Expressing, Marker